Hoechst is a hydrophobic dye that may freely cross an in tact
membrane and intercalate into the DNA. Hoechst fluoresces a blue
color when excited by UV and may therefore reveal the DNA morphology, therefore condensed
and intensely stained DNA and DNA fragmentation revealed by Hoechst are used as qualitative indications of apoptosis.
The following images were taken by Hashem A. Dbouk on 16/08/2006. The cell types used are BC3 and
BCBL1 (KSHV-infected cells, from normal patient and from HIV patient respectively), after treatment with As, IFN, As/IFN,
Special thanks to Youmna Kfoury, Ghada AbouLteif, and Hilda Al-Khoury.