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Hoechst Staining

Hoechst is a hydrophobic dye that may freely cross an in tact membrane and intercalate into the DNA. Hoechst fluoresces a blue color when excited by UV and may therefore reveal the DNA morphology, therefore condensed and intensely stained DNA and DNA fragmentation revealed by Hoechst are used as qualitative indications of apoptosis.
 
The following images were taken by Hashem A. Dbouk on 16/08/2006. The cell types used are BC3 and BCBL1 (KSHV-infected cells, from normal patient and from HIV patient respectively), after treatment with As, IFN, As/IFN, and PS341.
 
Special thanks to Youmna Kfoury, Ghada AbouLteif, and Hilda Al-Khoury.

BC3
bc3cntrl24.jpg
control 24 hours
BC3
bc3as48.jpg
As 48 hours
BC3
bc3ifn48.jpg
IFN 48 hours
BC3
bc3asif24.jpg
As/IFN 24 hours
BC3
bc3ps24.jpg
PS341 24 hours
BCBL1
bcbl1asifn24.jpg
As/IFN 24 hours
BCBL1
bcbl1asifn48.jpg
As/IFN 48